Tissue Culture Room Fumigation

Immediately exit the room.

Tissue culture room fumigation.

For this formaldehyde is used in combination with potassium permanganate. I went through the paper 38859pdf pdf calculation says that 1972gm of kmno4 and 3584 cc of formalin for each room. Add 35 ml formalin 37 or 40 formaldhyde to 10 g potassium permanganate per cubic metre of space in a porceline or steel container. Read the msds and perform a thorough risk assessment before fumigating the lab.

Formaldehyde vapour is very useful for fumigating room and incubators. Concentration exposure min calcium hypochlorite. My cultural lab size is 2987 cubic fit and growth chamber is of same size. Half fill the contaminated flask with 10 sodium hypochlorite.

Heating boiling 91 paraformaldehyde at the. Use the microscope to examine all tissue culture flasks for any contamination tiny dots of bacteria or stings of hyphae from fungi mould. Rooms à fumigation uv radiation. Dealing with cell culture contamination 1.

Laminar airflow hood à uv radiation. Remove all infected flasks into an appropriate laboratory where no tissue culture occurs. People working with bacteria and fungal cultures are not allowed to work in plant tissue culture laboratory including laminar flow usage and keeping the cultures also. The lab must be sealable.

Commonly used disinfectants for surface sterilization of the explant in plant tissue culture.