Tissue Culture Room Rules

Room air which is exhausted to a common plenum is not acceptable.

Tissue culture room rules.

All cell lines have to be tested for mycoplasma and re tested every 3 months 3. Mcb tissue culture room information rules. Completed all eh s required training online course offered by eh s http www ted uci edu laboratory core safety. Ensure there are no open doors or windows.

The latter approach minimizes entry exit from the hood in order to reduce disturbances in the laminar flow at the hood entrance as these may create. The do s of cell culture. Users must notify cell culture staff of their intention to work in the cell culture room s in order to receive specific instructions and to have assigned spaces fridge and freezer 2. Users names must be listed in both protocols and scrc listed in the ibc.

Contact the tc room to in charge to take a basic training course in cell culture techniques which involve viewing a tissue culture training video technique demonstration by the to in charge followed by a short written test. Do not leave without making sure that these conditions are met. Always leave the tissue culture tc room door locked. All room air shall be exhausted through ducts to the outside of the building and not recirculated within the building.

However some of them are specific to tissue culture. Cell culture best practices. Provides investigators with a sterile environment to work with and maintain bsl 1 organisms. No mycoplasma positive cell line is allowed in the tissue culture facility with exception of very rare and.

Tissue culture rules 1. Provide hscro and ibc. Procedure for using the culture room tissue culture room rules. Follow these simple rules to ensure healthy and reproducible cell cultures.

Make certain the gas line is shut off. Requirements for tissue culture rooms. If you are the last person to use the culture facility. Use personal protective equipment ppe laboratory coat gown gloves and eye protection at all times.

Check to make certain that the door of the incubator is securely latched and that the temperature is 37 0c and the co2 level is correct. Reduce foot traffic into the room as much as possible. Put on a lab coat and gloves. Attended the technique course or trained by approved labs or users.

Discard trash immediately or collect it in a small containable pile that you periodically empty. Tissue culture room checklist after entering the tissue culture room. Air shall flow from the hallway to the inner lab negative to the hallway. The tissue culture room is equipped with a laminar flow hood centrifuge water bath and two stackable co.

Ideally lab coats should live in the room and never be removed. Put all biologically contaminated tissue culture plates flasks and other non sharps in the non sharps biohazard waste container near the sink. In addition thermally insulated gloves full face visor and splash proof apron should be worn when. Incubators to maintain cell cultures.

Do not open the tc room door if it is in use displayed by the occupied sign.